Coding
colicin

Part:BBa_K896937:Design

Designed by: Shao-Lun Chen   Group: iGEM12_NYMU-Taipei   (2012-04-26)

colicin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primers used in the PCR: 

colicin_FP: gcttctag atgagcggtggcgatg(TM:56C)
colicin_RP: gct ctgcagcggccgctactagta ttacttaccccgatgaatatcaatatgt (TM:56C)

Digested the PCR product with XbaI and PstI, and ligated the digestion product with a gel purified XbaI,PstI digested pSB1A2 plasmid backbone.

Source

pIGMS5, a new plasmid from clinical Escherichia coli strain

References

Smorawinska,M. and Plucienniczak,A. Submitted (24-AUG-2006) Bioengineering, Institute of Biotechnology and Antibiotics, Staroscinska 5, Warsaw 02-516, Poland